Analysis Note

ControlIn fetal tissues, use spleen, brain stem and lung. In most adult tissues, use lymphoid tissues.

Application

Research Sub CategoryTranscription Factors

Western blot using ECL

Immunohistochemistry

Immunocytochemistry

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Research CategoryEpigenetics & Nuclear Function

Anti-Notch 1 Antibody, clone mN1A is a high quality Mouse Monoclonal Antibody for the detection of Notch 1 & has been validated in ICC, IHC, IP & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Notch1 consists of three domains. The extracellular domain containing 36 epidermal growth factor (EGF) repeats and 3 lin-12/Notch repeats (LNR) and functions as a receptor in cell-cell interactions. There is a single transmembrane domain (TM). The intracellular domain contains six cdc10/ankyrin repeats (cdc10) and the newly defined Notch cytokine response (NCR) region, which contains a putative bipartite NLS (nuclear localization signal).

Immunogen

Cdc10-NCR region of mouse Notch1 expressed as a GST fusion protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1046

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified immunoglobulin. Liquid.

Format: Purified

Specificity

Recognizes Notch1, intracellular domain. The antibody has a high affinity for the "activated" intracellular form of Notch1, but a low affinity for the full-length (unprocessed or heterodimeric cell surface) forms. No reactivity with Notch2, 3 or 4.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

272.5 kDa (also 230 kDa processed form)